关键词: 恒河猴, 脑脊液, 动态采集, 血常规, 电解质

Abstract: Objective　To establish a dynamic cerebrospinal fluid (CSF) collection model in rhesus monkeys, providing methodological support for pharmacokinetic studies of central nervous system drugs. Methods　Three adult rhesus monkeys were selected and subjected to lumbar puncture (L3-L4/L4-L5 intervertebral space) under anesthesia. After confirming CSF outflow, an indwelling catheter was inserted and secured. During the 72-hour catheter retention period, CSF samples were dynamically collected at 0, 12, 24, 48, and 72 hours for electrolyte and routine blood tests.Results　The lumbar catheter method  enabled multiple collections at five time points, with no significant differences in routine blood parameters (WBC, RBC, HGB, etc.) across time points. Electrolyte analysis revealed a statistically significant difference only in Cl- between 0 h and 12 h (P<0.05), while other indicators (K+, Na+, etc.) remained stable.Conclusion　The lumbar puncture catheter indwelling method established in this study is characterized by its simplicity, sample stability, and high reproducibility, effectively meeting the demands of dynamic CSF collection. It provides a reliable technical solution for studying drug metabolism in the central nervous system.

Key words: Rhesus monkey, cerebrospinal fluid, dynamic collection, routine blood test, electrolytes